Principal chondrocytes were cultured while in the presence or absence of IL-1? (10?ng/mL) for 24?h. RNA was gathered as mentioned above. Samples had been geared up according to library kit maker’s protocol, indexed, pooled, and sequenced on an Illumina HiSeq. Basecalls and demultiplexing ended up done with Illumina’s bcl2fastq program and a cus
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